I'm not sure if this will work, depending on what operating system you're running, but if you go to 'Control Panel' and then 'Sounds and Audio Devices' in Windows XP then click on the 'Hardware' tab it should bring up a list of all the hardware for audio things you've got on your computer. If you click on the name of your audio device to see the status and try trouble shooting it. Maybe even just try switching it off and enabling it again.
If it's not listed then something has probably gone wrong with your drivers for your soundcard. They may have got deleted somehow. If you know the make of the soundcard then try Googling the make and model and add "drivers" to the end of the search. A lot of these companies have the drivers to download from their website. If you can find it then it's just a case of downloading and reinstalling it (they're usually quite small files).
I'm not sure if that will help as I'm not particularly computer savvy myself but I've had similar issues before. I hope you get it sorted before your VJ gig tonight.
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